NG2 and GFAP co-expression after differentiation in cells transfected with mutant GFAP and in undifferentiated glioma cells. / Coexpresión de NG2/GFAP tras la diferenciación en células transfectadas con las mutaciones de GFAP y en células procedentes de gliomas indiferenciados.

INTRODUCTION: Alexander disease is a rare disorder caused by mutations in the gene coding for glial fibrillary acidic protein (GFAP). In a previous study, differentiation of neurospheres transfected with these mutations resulted in a cell type that expresses both GFAP and NG2. OBJECTIVE: To determine the effect of molecular marker mutations in comparison to undifferentiated glioma cells simultaneously expressing GFAP and NG2. METHODS: We used samples of human glioblastoma (GBM) and rat neurospheres transfected with GFAP mutations to analyse GFAP and NG2 expression after differentiation. We also performed an immunocytochemical analysis of neuronal differentiation for both cell types and detection of GFAP, NG2, vimentin, Olig2, and caspase-3 at 3 and 7 days from differentiation. RESULTS: Both the cells transfected with GFAP mutations and GBM cells showed increased NG2 and GFAP expression. However, expression of caspase-3-positive cells was found to be considerably higher in transfected cells than in GBM cells. CONCLUSIONS: Our results suggest that GFAP expression is not the only factor associated with cell death in Alexander disease. Caspase-3 expression and the potential role of NG2 in increasing resistance to apoptosis in cells co-expressing GFAP and NG2 should be considered in the search for new therapeutic strategies for the disease.

Morphologic Features on MR Imaging Classify Multifocal Glioblastomas in Different Prognostic Groups.

BACKGROUND AND PURPOSE: Multifocal glioblastomas (ie, glioblastomas with multiple foci, unconnected in postcontrast pretreatment T1-weighted images) represent a challenge in clinical practice due to their poor prognosis. We wished to obtain imaging biomarkers with prognostic value that have not been found previously. MATERIALS AND METHODS: A retrospective review of 1155 patients with glioblastomas from 10 local institutions during 2006-2017 provided 97 patients satisfying the inclusion criteria of the study and classified as having multifocal glioblastomas. Tumors were segmented and morphologic features were computed using different methodologies: 1) measured on the largest focus, 2) aggregating the different foci as a whole, and 3) recording the extreme value obtained for each focus. Kaplan-Meier, Cox proportional hazards, correlations, and Harrell concordance indices (c-indices) were used for the statistical analysis. RESULTS: Age (P < .001, hazard ratio = 2.11, c-index = 0.705), surgery (P < .001, hazard ratio = 2.04, c-index = 0.712), contrast-enhancing rim width (P < .001, hazard ratio = 2.15, c-index = 0.704), and surface regularity (P = .021, hazard ratio = 1.66, c-index = 0.639) measured on the largest focus were significant independent predictors of survival. Maximum contrast-enhancing rim width (P = .002, hazard ratio = 2.05, c-index = 0.668) and minimal surface regularity (P = .036, hazard ratio = 1.64, c-index = 0.600) were also significant. A multivariate model using age, surgery, and contrast-enhancing rim width measured on the largest foci classified multifocal glioblastomas into groups with different outcomes (P < .001, hazard ratio = 3.00, c-index = 0.853, median survival difference = 10.55 months). Moreover, quartiles with the highest and lowest individual prognostic scores based on the focus with the largest volume and surgery were identified as extreme groups in terms of survival (P < .001, hazard ratio = 18.67, c-index = 0.967). CONCLUSIONS: A prognostic model incorporating imaging findings on pretreatment postcontrast T1-weighted MRI classified patients with glioblastoma into different prognostic groups.

Histological changes in the rat brain and spinal cord following prolonged intracerebroventricular infusion of cerebrospinal fluid from amyotrophic lateral sclerosis patients are similar to those caused by the disease. / La infusión intracerebroventricular prolongada de líquido cefalorraquídeo procedente de pacientes con esclerosis lateral amiotrófica provoca cambios histológicos en el cerebro y la médula espinal de la rata similares a los hallados en la enfermedad.

INTRODUCTION: Cerebrospinal fluid (CSF) from amyotrophic lateral sclerosis (ALS) patients induces cytotoxic effects in in vitro cultured motor neurons. MATERIAL AND METHODS: We selected CSF with previously reported cytotoxic effects from 32 ALS patients. Twenty-eight adult male rats were intracerebroventricularly implanted with osmotic mini-pumps and divided into 3 groups: 9 rats injected with CSF from non-ALS patients, 15 rats injected with cytotoxic ALS-CSF, and 4 rats injected with a physiological saline solution. CSF was intracerebroventricularly and continuously infused for periods of 20 or 43days after implantation. We conducted clinical assessments and electromyographic examinations, and histological analyses were conducted in rats euthanised 20, 45, and 82days after surgery. RESULTS: Immunohistochemical studies revealed tissue damage with similar characteristics to those found in the sporadic forms of ALS, such as overexpression of cystatinC, transferrin, and TDP-43 protein in the cytoplasm. The earliest changes observed seemed to play a protective role due to the overexpression of peripherin, AKTpan, AKTphospho, and metallothioneins; this expression had diminished by the time we analysed rats euthanised on day 82, when an increase in apoptosis was observed. The first cellular changes identified were activated microglia followed by astrogliosis and overexpression of GFAP and S100B proteins. CONCLUSION: Our data suggest that ALS could spread through CSF and that intracerebroventricular administration of cytotoxic ALS-CSF provokes changes similar to those found in sporadic forms of the disease.

Suboptimal interventional conditions for the occlusion of ruptured intracranial aneurysms do not increase periprocedural complications and poor clinical outcomes.

BACKGROUND AND OBJECTIVE: Occluding a ruptured intracranial aneurysm as early as possible may entail certain periprocedural conditions that compromise the outcome. The aim of the present study was to evaluate the effectiveness, safety, and clinical outcome of endovascular coiling procedures performed on an emergency basis under potentially suboptimal conditions, and to compare results with those from scheduled procedures under potentially optimal conditions. METHODS: Interventions performed on 66 SAH patients were retrospectively analysed by classifying them into two groups: under emergency (within three hours from diagnosis or during non-standard working hours) or scheduled conditions. A binary logistic regression analysis was also performed to identify characteristics associated with poor outcomes. RESULTS: No differences in effectiveness, periprocedural complications, or clinical outcomes were found between the two groups. Rebleeding was detected in 4.8 % of the emergency interventions and 2.2 % of the scheduled interventions. Multivariate analysis identified age and Hunt and Hess grade, but no conditions of treatment, as the factors associated to poor outcome. CONCLUSION: Suboptimal interventional conditions for occluding ruptured intracranial aneurysms, such as performing procedures outside of standard working hours or within three hours of diagnosis, do not result in increased periprocedural complications and poor clinical outcomes compared with scheduled procedures under potentially optimal conditions. These results suggest the need for treatment to be provided as soon as possible.

[Cerebrospinal fluid cytotoxicity in lateral amyotrophic sclerosis]. / La citotoxicidad del líquido cefalorraquídeo en la esclerosis lateral amiotrófica.

INTRODUCTION: The cytotoxicity of cerebrospinal fluid (CSF) in patients with lateral amyotrophic sclerosis in cell cultures that include neurons may be considered as a diffusion mechanism of the disease, due to the proximity of the CSF to the spinal column. DEVELOPMENT: Various literature studies suggest that the motor neurons are more susceptible to cytotoxicity compared to other neuron cells, including glial, in cell cultures. The review of the composition of CSF in lateral amyotrophic sclerosis gives few clues on how this mechanism causes pre-apoptotic and apoptotic changes on the addition on CSF to the cultures, although it could be associated with the glutamate receptors, to a greater extent in those that respond to AMPA/kainate, and have a role in ion channels. CONCLUSIONS: The cytotoxicity of CSF is a peculiarity of lateral amyotrophic sclerosis, which could explain some aspects of how the disease progresses. More studies are required in order to understand more about this mechanism, including better identification of patients from whom samples are obtained, as well as their characteristics, differentiating them into familial or sporadic.

La citotoxicidad del líquido cefalorraquídeo en la esclerosis lateral amiotrófica / Cerebrospinal fluid cytotoxicity in lateral amyotrophic sclerosis

Introducción: La citotoxicidad del líquido cefalorraquídeo (LCR) de pacientes con esclerosis lateral amiotrófica en cultivos celulares que incluyen neuronas puede plantearse como un mecanismo de difusión de la enfermedad, debido a la cercanía del LCR a la médula espinal. Desarrollo: Los diferentes estudios de la literatura indican una mayor susceptibilidad del efecto citotóxico en las motoneuronas, frente a otro tipo de células neuronales y la inclusión de glía en los cultivos. La revisión de la composición del LCR en la esclerosis lateral amiotrófica no permite indicar mediante qué mecanismo se producen cambios preapoptóticos y apoptóticos con la adición del LCR a los cultivos, aunque podría estar relacionado con los receptores del glutamato, en mayor medida, aquellos que responden a AMPA/kainato, e intervenir en canales iónicos. Conclusiones: La citotoxicidad del LCR es una singularidad de la esclerosis lateral amiotrófica que podría explicar aspectos evolutivos de la enfermedad. Para el mejor conocimiento de este mecanismo, es necesario que nuevos estudios incluyan una mayor identificación de los pacientes de quienes se obtienen las muestras, así como sus características, y diferenciar si son formas familiares o esporádicas (AU)

Introduction: The cytotoxicity of cerebrospinal fluid (CSF) in patients with lateral amyotrophic sclerosis in cell cultures that include neurons may be considered as a diffusion mechanism of the disease, due to the proximity of the CSF to the spinal column. Development: Various literature studies suggest that the motor neurons are more susceptible to cytotoxicity compared to other neuron cells, including glial, in cell cultures. The review of the composition of CSF in lateral amyotrophic sclerosis gives few clues on how this mechanism causes pre-apoptotic and apoptotic changes on the addition on CSF to the cultures, although it could be associated with the glutamate receptors, to a greater extent in those that respond to AMPA/kainate, and have a role in ion channels. Conclusions: The cytotoxicity of CSF is a peculiarity of lateral amyotrophic sclerosis, which could explain some aspects of how the disease progresses. More studies are required in order to understand more about this mechanism, including better identification of patients from whom samples are obtained, as well as their characteristics, differentiating them into familial or sporadic (AU)

[Analysis of judicial sentences against neurosurgeons resolved in second court of justice in Spain in the period from 1995 to 2007]. / Análisis de las sentencias judiciales contra neurocirujanos resueltas en segunda instancia en España entre 1995 y 2007.

OBJECTIVES: With the aim of identifying the factors related to sentences against neurosurgeons, we have analyzed all the sentences issued in the second court of justice in Spain against neurosurgeons in the period from 1995 to 2007. MATERIAL AND METHODS: Of a total of 1899 sentences of the second or last appeal, during the period from 1995 to 2007 issued in Spain, 61 were chosen which fulfill the criteria to be included in our study. 25 variables were included on the record of compiled data. A complete descriptive and comparative study was elaborated, as well as an analysis of the type of suits, circumstances, and professionals involved. RESULTS: In a third of the cases, complete malpraxis was identified. In regards to the sentences, they were absolved in approximately half the cases, resolved with one fifth of the cases being penal, and four fifths with compensation. Indemnity quantities range from 60,000 to 600,000euro. Deficiency of information or consent was noted in 17% of lawsuits. 62.5% of operations in our sample were on the anatomic region of the vertebral column followed in frequency by the cranial region with 28.6%. Consequences of surgical procedure included major permanent sequelae in 40% of the cases and death in 22%. CONCLUSIONS: It is wise to invest time to deal with patients, including the verbal informed consent, which must be confirmed by the written informed consent form. It is also important to leave a written proof of medical praxis, both related to surgical records and to diagnosis and follow-up of the patient. Procedures with a lower life-threatening risk should not be underestimated, since they comprise the greatest demanded group. The greatest amount of demands is related to economic reimbursement, especially in private practice.

Análisis de las sentencias judiciales contra neurocirujanos resueltas en segundainstancia en España entre 1995 y 2007 / Analysis of Judicial Sentences Against Neurosurgeons Resolved in Second Court of Justice in Spain in the Period from 1995 to 2007

Objetivos. Con el fin de identificar los factoresrelacionados con las condenas judiciales contra neurocirujanos,el presente estudio analiza todas las sentenciasemitidas en segunda instancia en España contraneurocirujanos en el periodo de 1995 al 2007.Material y método. De un total de 1899 sentencias desegunda instancia o última durante el periodo de 1995a 2007 emitidas en España se han escogido 61 que cumplenlos criterios de inclusión para nuestro estudio. Sehan cumplimentado 25 variables en la ficha de recogidade datos. Se ha realizado con ellas un completo estudiodescriptivo y comparativo, además de un análisis de latipología de las demandas, las circunstancias y los profesionalesimplicados.Resultados. En un tercio de los casos se apreció completamal praxis. En relación a las condenas, se absolvióaproximadamente en la mitad de los casos, siendo lasresueltas con condena 1/5 de ellas de carácter penaly 4/5 indemnizatorias. Las cuantías indemnizatoriaspredominan en el rango de 60.000 a 600.000 euros.En un 17% de las demandas se apreció deficiencia enla información o el consentimiento. El 62,5% de lasintervenciones de nuestra muestra se produjeron en laregión anatómica de la columna vertebral siguiéndoleen frecuencia la región craneal con un 28,6%. De lasconsecuencias del procedimiento quirúrgico las secuelaspermanentes mayores constituyen el 40% de loscasos y el fallecimiento el 22%.Conclusiones. Es rentable dedicar tiempo a la relacióncon el paciente, incluyendo el consentimiento informadoverbal, que debe ser refrendado por la (..) (AU)

Objectives. With the aim of identifying the factorsrelated to sentences against neurosurgeons, we haveanalyzed all the sentences issued in the second courtof justice in Spain against neurosurgeons in the periodfrom 1995 to 2007.Material and methods. Of a total of 1899 sentences ofthe second or last appeal, during the period from 1995to 2007 issued in Spain, 61 were chosen which fulfill thecriteria to be included in our study. 25 variables wereincluded on the record of compiled data. A completedescriptive and comparative study was elaborated, aswell as an analysis of the type of suits, circumstances,and professionals involved.Results. In a third of the cases, complete malpraxiswas identified. In regards to the sentences, they wereabsolved in approximately half the cases, resolved withone fifth of the cases being penal, and four fifths withcompensation. Indemnity quantities range from 60,000to 600,000€. Deficiency of information or consent wasnoted in 17% of lawsuits. 62.5% of operations in oursample were on the anatomic region of the vertebralcolumn followed in frequency by the cranial regionwith 28.6%. Consequences of surgical procedure includedmajor permanent sequelae in 40% of the cases anddeath in 22%.Conclusions. It is wise to invest time to deal withpatients, including the verbal informed consent, whichmust be confirmed by the written informed consent form.It is also important to leave a written proof of (..) (AU)

Estimulación cerebral profunda en los trastornos del humor: ¿tan fácil como parece? / Deep brain stimulation in mood disorders: is it as easy as it seems?

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[Experimental models of traumatic brain injury]. / Modelos experimentales de traumatismo craneoencefálico.

AIM: To provide a summary of the different experimental models of traumatic brain injury (TBI) designed under both in vivo and in vitro conditions. A comprehensible review of the specific types of brain lesions induced, as well as the technical details to reproduce each model at the laboratory is given. DEVELOPMENT: Outcome of patients suffering from a TBI has significantly improved with the rapid application of vital supporting measures in addition to a strict control of blood and intracranial pressure at the intensive care units. However no specific treatment for post-traumatic brain lesions has proven as efficacious in the clinical settings. A deeper knowledge of the physiopathological events associated with TBI is necessary for the development of new specific therapies. Due to the heterogeneity of the human TBI, each experimental model has been designed to reproduce a different type of brain lesion. Experimental TBI models allow the study of the dynamic evolution of brain injuries under controlled conditions. Usefulness of experimental models is limited by their reliability and reproducibility among different researchers. Small rodents have been the preferred animals to reproduce TBI injuries, mainly due to the similar cerebral physiology shared by these animals and the human beings. CONCLUSION: The use of experimental models of TBI is the most appropriate tool to study the mechanisms underlying this type of injury. However their simplicity precludes an exact reproduction of the heterogeneous cerebral damage observed in clinical settings. This could be the main reason for the discrepancies observed in the therapeutic effects of treatments between experimental and clinical studies.

Inhibition of adult hippocampal neurogenesis disrupts contextual learning but spares spatial working memory, long-term conditional rule retention and spatial reversal.

Neurogenesis in the adult dentate gyrus (DG) of the hippocampus has been implicated in neural plasticity and cognition but the specific functions contributed by adult-born neurons remain controversial. Here, we have explored the relationship between adult hippocampal neurogenesis and memory function using tasks which specifically require the participation of the DG. In two separate experiments several groups of rats were exposed to fractionated ionizing radiation (two sessions of 7 Gy each on consecutive days) applied either to the whole brain or focally, aiming at a region overlying the hippocampus. The immunocytochemical assays showed that the radiation significantly reduced the expression of doublecortin (DCX), a marker for immature neurons, in the dorsal DG. Ultrastructural examination of the DG region revealed disruption of progenitor cell niches several weeks after the radiation. In the first experiment, whole-brain and focal irradiation reduced DCX expression by 68% and 43%, respectively. Whole-brain and focally-irradiated rats were unimpaired compared with control rats in a matching-to-place (MTP) working memory task performed in the T-maze and in the long-term retention of the no-alternation rule. In the second experiment, focal irradiation reduced DCX expression by 36% but did not impair performance on (1) a standard non-matching-to-place (NMTP) task, (2) a more demanding NMTP task with increasingly longer within-trial delays, (3) a long-term retention test of the alternation rule and (4) a spatial reversal task. However, rats irradiated focally showed clear deficits in a "purely" contextual fear-conditioning task at short and long retention intervals. These data demonstrate that reduced adult hippocampal neurogenesis produces marked deficits in the rapid acquisition of emotionally relevant contextual information but spares spatial working memory function, the long-term retention of acquired spatial rules and the ability to flexibly modify learned spatial strategies.

Muerte neuronal en la esclerosis lateral amiotr¨®fica / Neuronal death in amyotrofic lateral sclerosis

Introducci¨®n. El reciente fracaso del ensayo cl¨ªnico de minociclinaplantea si el mecanismo de desarrollo de la muerteneuronal en la esclerosis lateral amiotr¨®fica espor¨¢dica (SALS)es diferente al que ocurre en los modelos de rat¨®n transg¨¦nicocon mutaciones humanas relacionadas con SOD (TgALS).M¨¦todo. Existen diferencias entre los mecanismos deestr¨¦s oxitativo en TgALS y SALS de perfil e intensidad. Mientrasla apoptosis en TgALS procede primariamente de la mitocondriay conduce a la activaci¨®n de caspasa 9 previa descargade Bid y citocromo C, en la SALS si existiera apoptosisse podr¨ªa plantear la hip¨®tesis que pudiera proceder a trav¨¦sde la activaci¨®n de la v¨ªa del FAS, a trav¨¦s de la catepsina Bo del TNF-¦Á, que podr¨ªa provenir de la activaci¨®n de la microgliao con origen citoplasm¨¢tico. En la v¨ªa del FAS, elTNF-¦Á act¨²a como ligando del receptor FAS, lo que conducir¨¢a la activaci¨®n de caspasa 8, aunque la catepsina B podr¨ªaactuar directamente sobre ella. Considerando que laminociclina produce la disminuci¨®n de la descarga de citocromoC dificultando la producci¨®n de las caspasas ejecutorasa trav¨¦s de la v¨ªa intr¨ªnseca, se justifica su eficacia enTgALS, pero no as¨ª si en la apoptosis en la SALS si se confirmasu desarrollo primariamente en la v¨ªa del FAS.Conclusiones. Un mejor conocimiento de c¨®mo se producela muerte celular en la SALS puede sugerir opcionesterap¨¦uticas y discriminar entre aquellas que, aun mostrandoeficacia en TgALS, pueden no serlo en SALS (AU)

Introduction. The recent failure of the clinical trial of ninocycline outlin es if the mechanism of development of the death neuronal in the sporadic amyotrophic lateral sclerosis (SALS) is different to that happens in models of transgenic mice with human mutations related with SOD (TgALS). Method. Differences on profile and intensity exist among the oxidative stress mechanisms between TgALS and SALS. Whereas the origin of apoptosis pathway in TgALS comes from the mithocondria and drives to caspase 9 with previous Bid and citocrome C discharge, in SALS, if apoptosis exists, that could proceed through activation ofFAS pathway by means of cathepsin B, or ¦Á-TNF, for microgl¨ªa activation or from cell cytosol. In FAS pathway, TNF-¦Á acts receptor ligands what drives to caspase 8 activation, although cathepsine B could act directly. Considering that the minocycline decreases Citocrome discharge, reducing executors caspases expression proceeding from intrinsic pathway, is justified its effectiveness inTgALS, but could not be explained if apoptosis in SALS was developed primarily on FAS pathway.Conclusions. Better knowledge of how cellular death occurs in SALS, could allow to suggest therapeuticoptions, and could permit to discriminate drugs that, showing effectiveness in TgALS, could not be beneficialsin SALS (AU)

The hippocampal dentate gyrus is essential for generating contextual memories of fear and drug-induced reward.

The hippocampus is believed to play a role in processing information relative to the context in which emotionally salient experiences occur but evidence on the specific contribution of the hippocampal dentate gyrus (DG) to these processes is limited. Here, we have used two classical behavioral paradigms to study the participation of the dorsal DG in context-conditioned reward and context-conditioned fear. Rats received intra-hippocampal vehicle or colchicine injections (4 microg/microl solution; 0.2 microl injections at 10 sites) that damaged the DG but spared other hippocampal subfields. In the first experiment, we used a place conditioning procedure pairing cocaine exposure (20 mg/kg, i.p.) with a specific context and vehicle treatment with another. While rats with sham lesions exhibited preference for the cocaine-paired context following conditioning, rats with lesions of the DG showed no evidence of cocaine-induced place preference. In the second experiment, rats with sham or colchicine lesions received a foot shock in a given context and conditioned freezing was measured upon reexposure to the shock-paired context (2, 24, 48 and 96 h after conditioning). Rats with sham lesions exhibited high levels of conditioned freezing when exposed to the conditioning context but rats with lesions of the DG showed impaired conditioning, behaving as controls that had experienced shock in a different context. These observations indicate that the integrity of the DG is essential for establishing a coherent representation of the context to which emotional experiences, either hedonic or aversive, are bound.

[Cellular therapy in amyotrophic lateral sclerosis]. / Terapia celular en la esclerosis lateral amiotrófica.

INTRODUCTION: The possible role of stem cells transplantation in therapy for traumatic lesions or for diseases has been outlined in recent years. Amyotrophic lateral sclerosis (ALS) is one of the diseases where cellular therapy may be useful. DEVELOPMENT: The authors make an analytic review of the studies carried out in humans with ALS and in G93A transgenic rodent model of ALS to evaluate the effect of stem cell transplantation. They also review cellular responses from NSC-EZ cells in the spinal cord. CONCLUSIONS: Research on the potential uses of cellular therapy for ALS is on-going, however, the different studies are not homogeneous. Thus, many questions need to be answered, such as which is the most appropriate type of cells or which should be the volume of cells to implant, which is the best method for the transplantation and in the case of spinal cord implant which is the best target for the implant, or if it is necessary to administer concomitant substances, such as immunosuppressant drugs.

Primary immunoblastic B-cell lymphoma of the cranial vault.

We present the first reported patient with primary immunoblastic non-Hodgkin's lymphoma of the cranial vault, with a fatal outcome in spite of complete surgical removal.

[Present status of psychosurgery in Spain]. / Estado actual de la psicocirugía en España.

In order to know the present activity of psychosurgery in Spain, and the opinion of neurosurgeons relative to it, a survey was designed and applied to all active neurosurgeons in our country. We obtained data from at least one neurosurgeon from the 74 neurosurgical centers in Spain (response rate= 100%). Only 6 neurosurgeons performed psychosurgical interventions. In total, 121 psychosurgeries were performed between 1999 and 2003, 75.7% of them in private centers. The most frequent indication is obsessive-compulsive disorder and the most frequent technique is anterior capsulotomy, although techniques and indications differ among the practising neurosurgeons. Those not performing them cite lack of patient referral (54.4%) or unexperience (36.8%) as the causes. A suspected lack of efficacy or the possible adverse effects are seldom expressed. The possibility of using deep brain stimulation for psychiatric indications, as well as the experience of some neurosurgeons and the favorable opinion of the rest, might increase the number of operations in our country.

Modelos experimentales de esclerosis lateral amiotrófica / Experimental models of amyotrophic lateral sclerosis

Introducción. La esclerosis lateral amiotrófica (ELA) es una enfermedad neurodegenerativa grave que afecta de forma casi selectiva a las motoneuronas. Su etiopatogenia no se conoce totalmente en el momento actual, aunque se han implicado diversos mecanismos. No existe un tratamiento curativo para esta enfermedad y sólo un fármaco (riluzol) y la ventilación mecánica han demostrado tener algún efecto en la supervivencia de los pacientes que la padecen. En los últimas dos décadas el desarrollo de varios modelos experimentales ha permitido comprender mejor esta enfermedad y diseñar posibles tratamientos.Método. Revisamos los artículos publicados concernientes a modelos experimentales para ELA y para enfermedades neurodegenerativas utilizando la base de datos PubMed.Resultados. Se han descrito diversos modelos experimentales, desde animales (siendo los más frecuentes los animales transgénicos para mutaciones humanas de la superóxido dismutasa [SOD1]) hasta modelos celulares, cada uno de ellos con ventajas e inconvenientes.Conclusiones. Los modelos experimentales de la ELA han supuesto un gran avance en el conocimiento de esta enfermedad y en el diseño de nuevas estrategias terapéuticas

Introduction. Amyotrophic lateral sclerosis (ALS) is a serious neurodegenerative disease that affects almost selectively motor neurons. Its ethiopathogeny is not fully understood, although there are several mechanisms that could play a role. It has no curative treatment and just a drug (riluzole) and mechanical ventilation has demonstrated to improve survival of these patients. In the last decades experimental models have been developed which have led us to better understand this disease and to design possible therapeutic strategies.Method. We reviewed published articles concerning experimental models for ALS and neurodegeneratives diseases using the PubMed database.Results. Several experimental models for ALS have been described, from animal models (mainly transgenic animals for human mutations in superoxidedismutase [SOD1]) to cellular models, each of them with advantages and objections.Conclusions. ALS experimental models have implied a great advance in the knowledge of this disease and the design of new therapeutic strategies